Genome engineering empowers the diatom Phaeodactylum tricornutum for biotechnology - Diatom Genomics Access content directly
Journal Articles Nature Communications Year : 2014

Genome engineering empowers the diatom Phaeodactylum tricornutum for biotechnology

Fayza Daboussi
Sophie Leduc
  • Function : Author
Alan Marechal
  • Function : Author
Valerie Guyot
  • Function : Author
Christophe Perez-Michaut
  • Function : Author
Alberto Amato
Alexandre Juillerat
  • Function : Author
Marine Beurdeley
  • Function : Author
Daniel F. Voytas
  • Function : Author
Laurent Cavarec
  • Function : Author
Philippe Duchateau
  • Function : Author

Abstract

Diatoms, a major group of photosynthetic microalgae, have a high biotechnological potential that has not been fully exploited because of the paucity of available genetic tools. Here we demonstrate targeted and stable modifications of the genome of the marine diatom Phaeodactylum tricornutum, using both meganucleases and TALE nucleases. When nuclease-encoding constructs are co-transformed with a selectable marker, high frequencies of genome modifications are readily attained with 56 and 27% of the colonies exhibiting targeted mutagenesis or targeted gene insertion, respectively. The generation of an enhanced lipid-producing strain (45-fold increase in triacylglycerol accumulation) through the disruption of the UDP-glucose pyrophosphorylase gene exemplifies the power of genome engineering to harness diatoms for biofuel production.
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hal-01528413 , version 1 (28-05-2020)

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Fayza Daboussi, Sophie Leduc, Alan Marechal, Gwendoline Dubois, Valerie Guyot, et al.. Genome engineering empowers the diatom Phaeodactylum tricornutum for biotechnology. Nature Communications, 2014, 5, pp.3831. ⟨10.1038/ncomms4831⟩. ⟨hal-01528413⟩
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